Preservation of RNA for functional analysis of separated alleles in yeast: comparison of snap-frozen and RNALater solid tissue storage methods.

Author(s): Dekairelle AF, Van der Vorst S, Tombal B, Gala JL

Publication: Clin Chem Lab Med, 2007, Vol. 45, Page 1283-7

PubMed ID: 17727312 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare detection of p53 mutations by FASAY test in bladder carcinoma specimens stored either in RNAlater at room temperature or 4 degrees C or snap-frozen and stored at -80 degrees C.

   Summary of Findings:

   Identical results were obtained between snap-frozen and RNAlater-preserved specimens in 50 of 53 tumors. Of these, 10 tumors were identified by FASAY to be positive for p53 mutations in both specimens types. The 3 discrepant results came from 2 additional RNAlater-preserved specimens and 1 additional snap-frozen specimen that were positive for p53 mutations. All specimens found to be positive by FASAY were confirmed to have a p53 mutation by sequencing. Storage of RNAlater-preserved specimens at room temperature for 3 days or at 4 degrees C for up to one month allowed for successful p53 amplification in 92% of specimens. However, in RNAlater-preserved specimens, storage for longer than 1 month at 4 degrees C resulted in amplification failure in approximately 50% of specimens.

   Biospecimens

   • Tissue - Bladder

   Preservative Types

   • RNAlater
   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	RT-PCR
   RNA	FASAY

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	RNAlater Snap frozen
   Storage	Storage temperature	Room temperature 4 degrees C -80 degrees C
   Storage	Storage duration	3 months More than 3 months 1 d 2 d 3 d 4 d 5 d 6 d 7 d 8 d 9 d 10 d 11 d 12 d 13 d 14 d 15 d 1 month 2 months

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