Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effect of short- and long-term storage on human serum and recombinant apolipoprotein E concentration.

Author(s): Schiele F, Vincent-Viry M, Herbeth B, Visvikis A, Siest G

Publication: Clin Chem Lab Med, 2000, Vol. 38, Page 525-8

PubMed ID: 10987201 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage duration and temperature and freeze-thaw cycling on apo E levels in serum. Blood was collected from fasting healthy individuals into serum separator tubes. After centrifugation, serum was stored in polypropylene tubes in 1 (4, -20 or -80 degrees C) or 0.25 mL aliquots (-196 degrees C) or assayed within 1 h.

   Summary of Findings:

   Apo E levels were stable in serum stored for 1 week at 4 degrees C, but levels decreased by 6.6% after 2 weeks at 4 degrees C (p<0.001). When serum was stored at -20 or -80 degrees C, apo E concentrations were stable for at least 3 months. Further, apo E concentrations were stable for 4 years at -196 degrees C. Notably, apo E concentrations were unaffected by 15 freeze-thaw cycles in a three week period, but a non-significant 5.2% decline was noted after 15 cycles(p=0.06).

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)
   • Other Preservative
   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Lipoprotein	Immunoturbidometric assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	4 degrees C -20 degrees C -80 degrees C -196 degrees C
   Storage	Storage duration	1 h 1 week 2 weeks 1 month 3 months <1 years 2 years 4 years
   Storage	Freeze/thaw cycling	1 cycle 3 cycles 5 cycles 7 cycles 9 cycles 11 cycles 13 cycles 15 cycles
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh) Refrigeration

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