Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Development, validation and clinical evaluation of a dried urine spot method for determination of hippuric acid and creatinine.

Author(s): Antunes MV, Niederauer CG, Linden R

Publication: Clin Biochem, 2013, Vol. 46, Page 1276-80

PubMed ID: 23868019 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study investigated the effects of using dried urine spots rather than urine and storage of dried urine spots on the measured levels of creatinine and hippuric acid using HPLC-DAD. Aliquots of urine containing 630 mg/L creatinine and 200 mg/hippuric acid or 1200 mg/L creatinine and 2700mg/L hippuric acid were spotted onto Whatman paper and allowed to dry at room temperature for 3 h. Dried urine specimens were stored in sealed bags at 4, 25, and 40˚C for 1, 4, 7, and 11 days. Levels of creatinine and hippuric acid were also determined in 49 patients exposed to toluene. Levels of creatinine and hippuric acid were measured by placing 5 mm punches of the dried urine spot in purified water and vortexing before HPLC-DAD analysis.

   Summary of Findings:

   Urine volume was very strongly correlated to urine dried spot area (r=0.9981). When dried urine spots were used, the concentration of hippuric acid averaged 116% of that in urine and the concentration of creatinine averaged 89% of that in urine.  There was no effect of storing dried urine spots for 11 days at 4, 25, or 40˚C on levels of creatinine or hippuric acid, regardless of initial concentration. Although the hippuric acid to creatinine ratio in dried urine spots averaged 116% of that in urine, the risk classification of toluene exposure based on the hippuric acid to creatinine ratios was in agreement between matched dried urine spots and urine for all 49 patients. 

   Biospecimens

   • Bodily Fluid - Urine

   Preservative Types

   • Other Preservative
   • None (Fresh)

   Diagnoses:

   • Other diagnoses
   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	HPLC

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Biomarker level	630 mg/L creatinine and 200 mg/hippuric acid 1200 mg/L creatinine and 2700mg/L hippuric acid
   Storage	Storage temperature	4˚C 25˚C 40˚C
   Storage	Storage duration	1 day 4 days 7 days 11 days
   Biospecimen Preservation	Type of fixation/preservation	Air-dried None (fresh)

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