Interference by o-phenanthroline in the radioimmunoassay of angiotensin II in small volume blood samples.
Author(s): Braithwaite JE, Yandle TG, Nicholls MG, Lewis LK
Publication: Clin Biochem, 2012, Vol. 45, Page 168-70
PubMed ID: 22040809 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of enzyme inhibitor cocktail concentration on the measurement of Ang-II in blood. To determine the effects of inhibitor to blood ratio, 1, 2 or 3 mL aliquots of blood were mixed with 150 uL of enzyme inhibitor.
Summary of Findings:
Increasing the concentration of enzyme inhibitor cocktail by decreasing the blood volume from 3 mL to 1 mL led to a 3-fold increase in measured Ang-II levels (p<0.05). The authors found that adding O-phenanthroline to the buffer, but not the other components of the inhibitor cocktail, led to a marked increase in measured Ang-II.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Peptide Radioimmunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Analyte Extraction and Purification Protease inhibitor Cocktail
Multiple concentrations evaluated