Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Polymerase chain reaction: amplification of DNA from fixed tissue.

Author(s): Crisan D, Cadoff EM, Mattson JC, Hartle KA

Publication: Clin Biochem, 1990, Vol. 23, Page 489-95

PubMed ID: 2289306 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of formalin and B-5 fixation on the success rates of PCR for BM specimens. Fresh and frozen peripheral blood leukocyte specimens were used as controls.

   Summary of Findings:

   DNA was successfully amplified from all BM specimens fixed in either formalin or B-5. For some B-5-fixed specimens, a second PCR was necessary to improve product yield. Detection of PCR products by Southern transfer using ASO for the Beta-globin gene was also successful for all BM specimens.

   Biospecimens

   • Tissue - Bone Marrow
   • Cell - White Cells

   Preservative Types

   • Formalin
   • None (Fresh)
   • Other Preservative
   • Frozen

   Diagnoses:

   • Diabetes Type 1
   • Normal
   • Neoplastic - Lymphoma
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	B-5 fixative Formalin (buffered) Frozen None (fresh)
   Biospecimen Acquisition	Biospecimen location	Bone marrow Peripheral blood leukocytes
   Preaquisition	Patient genotype	Hemoglobin AA Hemoglobin AS Hemoglobin SS Hemoglobin SC
   Preaquisition	Diagnosis/ patient condition	Anemia Hodgkin's disease Renal failure

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