New storage procedure for human tumor biopsies prior to estrogen receptor measurement.

Author(s): Crawford D, Cowan S, Hyder S, McMenamin M, Smith D, Leake R

Publication: Cancer Res, 1984, Vol. 44, Page 2348-51

PubMed ID: 6722773 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare ER stability among paired biopsy specimens stored either in a sucrose:glycerol media at -20 degrees C or frozen in liquid nitrogen.

   Summary of Findings:

   ER status among cytosol and nuclear fractions was in agreement in 84% of differentially preserved biopsy specimens. Amounts of ER were also comparable among preservation methods.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Lowry protein assay
   Protein	Receptor binding
   DNA	Fluorometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-20 degrees C -80 degrees C
   Biospecimen Preservation	Cooling or freezing method/ rate	Liquid nitrogen Sucrose:glycerol media

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2. Study Purpose

   The purpose of this study was to determine if ER levels remain stable in biopsy specimens stored in sucrose:glycerol media at -20 degrees C for up to 212 days.

   Summary of Findings:

   ER levels were stable in biopsy specimens stored at -20 degrees C in sucrose:glycerol media for up to 96 days. Receptor loss was observed after 212 days of storage.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Receptor binding

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	10 d 40 d 96 d 212 d

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