Whole blood cryopreservation in epidemiological studies.
Author(s): Hayes RB, Smith CO, Huang WY, Read Y, Kopp WC
Publication: Cancer Epidemiol Biomarkers Prev, 2002, Vol. 11, Page 1496-8
PubMed ID: 12433734 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of whole blood storage on lymphocyte viability, T-cell proliferation, and Epstein-Barr virus (EBV) transformation of B-cells.
Conclusion of Paper
Lymphocyte viability was decreased after whole blood was frozen and stored for one day, but increasing amounts of storage time had no additional effect. The percentage of T-cells, and T-cell:B-cell ratios were unaffected by storage of whole blood for one day, but each increased with increasing storage duration. Further, T-cell proliferation decreased with longer storage. EBV-transformation was unaffected by frozen storage of whole blood for up to 20 months.
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage of whole blood on lymphocyte viability, T-cell proliferation, and EBV-transformation of B-cells.
Summary of Findings:
An average of 98.4% of lymphocytes separated from fresh whole blood were viable, but after whole blood was frozen and stored for one day, that average decreased to 85.2%. Additional frozen storage up to 31 months did not significantly decrease lymphocyte viability. Freezing whole blood specimens for 1 day did not affect the percentage of T-cells or T-cell:B-cell ratios, however, both increased significantly with longer storage. T-cell proliferation decreased with longer storage. After 2 attempts, 92.5% of specimens stored for up to 20 months were successfully transformed with EBV taking an average time of 38.2 days.
Biospecimens
Preservative Types
- Frozen
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Cell count/volume Flow cytometry Cell count/volume Macroscopic observation Cell count/volume Radioassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Anticoagulant Acid-citrate-dextrose
Storage Storage temperature -150 degrees C
Storage Storage duration 0 d
1 d
1 month
7 months
18 months
20 months
31 months
Biospecimen Preservation Type of fixation/preservation Frozen
None (fresh)
Biospecimen Aliquots and Components Blood and blood products Lymphocyte