Collection of Genomic DNA from Adults in Epidemiological Studies by Buccal Cytobrush and Mouthwash
Author(s): Garcia-Closas M, Egan K, Abruzzo J, Newcomb P, Titus-Ernstoff L, Franklin T, Bender P, Beck J, Le Marchand L, Lum A, Alavanja M, Hayes RB, Rutter J, Buetow KH, Brinton L, Rothman N
Publication: Cancer Epidemiol Biomarkers Prev, 2001, Vol. 10, Page 687
PubMed ID: 11401920 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
To compare cytobrush samples sent by mail at room temperature without a transport medium with alcohol-based mouthwash samples sent by mail at room temperature for self-collection of buccal cells in terms of total human DNA yield, DNA integrity, and PCR success in amplifying beta-actin gene fragments.
Summary of Findings:
Total and human DNA yields from cytobrush samples were substantially lower than yields from mouthwash samples. In addition, cytobrush and mouthwash samples contain a mixture of DNA of human and nonhuman origin, and the amount of human DNA was higher in the mouthwash samples.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform DNA Electrophoresis DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Method of fluid acquisition Cytological brush
Swishing
Biospecimen Acquisition Method of cell acquisition Cytological brush
Swishing
-
Study Purpose
To compare phenol-chloroform DNA extraction to QIAamp DNA Blood Mini Kit, Puregene DNA Isolation Kit, and NaOH extraction in terms of total human DNA yield, DNA integrity, and PCR success in amplifying beta-actin gene fragments.
Summary of Findings:
Total and human DNA yields from cytobrush or mouthwash samples extracted by the QIAamp blood kit (Qiagen Inc.) were lower than the yields obtained by a phenol-chloroform extraction method. In addition, QIAamp DNA extracts were of lower molecular weight than phenol-chloroform extracts. NaOH-extracted DNA was of low molecular weight and presented difficulties for PCR amplification of beta-globin gene fragments. Total and human DNA yields as well as DNA integrity, PCR success rates, and number of attempts for a successful amplification were very similar for mouthwash samples extracted by Puregene or by phenol-chloroform.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform DNA DNA sequencing DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Analyte Extraction and Purification Analyte isolation method Phenol-chloroform
QIAamp DNA Blood Mini Kit
Puregene DNA Isolation Kit
NaOH extraction