Quality control program for storage of biologically banked blood specimens in the Malmo Diet and Cancer Study.

Author(s): Pero RW, Olsson A, Bryngelsson C, Carlsson S, Janzon L, Berglund G, Elmståhl S

Publication: Cancer Epidemiol Biomarkers Prev, 1998, Vol. 7, Page 803-8

PubMed ID: 9752989 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of cryopreservation on the quality and viability of plasma, serum, granulocytes, erythrocytes, and MNL.

   Summary of Findings:

   Plasma, serum, granulocytes and erythrocytes stored at -80 degrees C for 137-190 weeks did not have significant oxidative stress or DNA damage. However, storage of purified MNL at -140 degrees C for longer than 40 weeks resulted in declines of around 95% in proliferative capacity (p<0.05). Although freezing MNL at -140 degrees C resulted in a 30% decrease in viability compared to fresh MNL, no further decrease in viability was seen between 1 week and 128 weeks of storage. MNL isolated from cryopreserved buffy coats maintained a proliferative response however the authors performed this analysis using buffy coats that had been stored at -140 degrees C for less than 1 month and compared the proliferative response to MNL that had been stored at -140 degrees C for longer than 100 weeks. The authors recommend cryopreservation of buffy coats rather than purified MNL and granulocytes.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Cell - Blood
   • Cell - White Cells
   • Cell - Red Blood Cells
   • Bodily Fluid - Buffy Coat

   Preservative Types

   • Frozen
   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Small molecule	Spectrophotometry
   Small molecule	HPLC
   Cell count/volume	Lymphocyte proliferation assay
   Cell count/volume	Light microscopy
   DNA	Nucleoid sedimentation assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Anticoagulant	Heparin None
   Biospecimen Aliquots and Components	Blood and blood products	Buffy coat Leukocyte Plasma Serum Erythrocyte Granulocyte
   Storage	Storage temperature	-80 degrees C -140 degrees C
   Storage	Storage duration	0-190 weeks
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh)

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