NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Validation of a tissue microarray to study differential protein expression in inflammatory and non-inflammatory breast cancer.

Author(s): Van den Eynden GG, Van der Auwera I, Van Laere S, Colpaert CG, van Dam P, Merajver S, Kleer CG, Harris AL, Van Marck EA, Dirix LY, Vermeulen PB

Publication: Breast Cancer Res Treat, 2004, Vol. 85, Page 13-22

PubMed ID: 15039594 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine if immunohistochemical (IHC) staining of tissue microarray (TMA) cores can be used to characterize inflammatory breast cancer (IBC).

Conclusion of Paper

In 50% of IBC cases, at least one of the 5 cores was non-informative due to specimen loss or lack of tumor cells , but only 4.9% of non-IBC cases had non-informative cores. The number of informative cores was modestly but significantly lower among TMA sections that underwent heat-mediated antigen retrieval (30 minutes in a 98°C water bath) compared to those that did not. The concordance rates of estrogen receptor (ER), progesterone receptor (PR), human epidermal growth factor receptor 2 (HER-2) and p53 immunostaining in the TMA to those in the pathology reports based on whole section analysis were 0.74, 0.90, 0.85 and 0.50, respectively. The TMA confirmed the differential expression of ER, PR, HER-2, p53, RhoC GTPase, and E-cadherin between IBC and non-IBC specimens, but it did not identify any differences in expression of epidermal growth factor receptor (EGFR) or carbonic anhydrous IX (CA IX).

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of staining TMAs rather than entire sections for the analysis of IBC and non-IBC specimens. The array contained 5 cores for each of 34 IBC cases and 3 cores for each of 41 non-IBC cases. ER and PR staining was scored on a scale of 0-3, HER-2 on a scale of 0-2, and p53 as positive (10% or more of tumor cells staining) or negative.

    Summary of Findings:

    In 17 of 34 IBC cases (50%), at least one of the 5 cores was non-informative due to specimen loss or lack of tumor cells, and 8 of the 34 (23.5%) cases had no informative cores. Only 2 of 41 non-IBC cases had non-informative cores, one of which had no informative cores. The number of informative cores was modestly but significantly lower among TMA sections that underwent heat-mediated antigen retrieval (30 minutes in a 98°C water bath) compared to those that did not (P<0.015). The concordance rates of ER, PR, HER-2 and p53 immunostaining in the TMA to those in the pathology reports based on whole section analysis were 0.74, 0.90, 0.85 and 0.50, respectively. A high degree of correlation in HER-2 staining was found between specimens stained using the HercepTest and the CB11, TAB250, or CB11 and TAB250 antibodies (k=0.86, 0.78, and 0.83, respectively). The TMA confirmed the differential expression of ER, PR, HER-2, p53, RhoC GTPase, and E-cadherin between IBC and non-IBC specimens, but it did not identify any differences in expression of EGFR or CA IX.

    Biospecimens
    Preservative Types
    • Formalin
    Diagnoses:
    • Not specified
    Platform:
    AnalyteTechnology Platform
    Protein Immunohistochemistry
    Protein Tissue microarray
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Aliquots and Components Type of slide Whole tissue section
    Tissue microarray
    Immunohistochemistry Specific Targeted peptide/protein ER
    PR
    HER-2
    p53
    E-cadherin
    EGFR
    RhoC GTPase
    CA IX
    Immunohistochemistry Specific Type of antibody HercepTest
    TAB250
    CB11
    Both TAB250 and CB11
    Preaquisition Diagnosis/ patient condition Inflammatory breast cancer
    Non-inflammatory breast cancer
    Analyte Extraction and Purification Antigen retrieval 30 min at 98 degrees C
    None
    Antigen retrieval solution

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