Validation of a tissue microarray to study differential protein expression in inflammatory and non-inflammatory breast cancer.

Author(s): Van den Eynden GG, Van der Auwera I, Van Laere S, Colpaert CG, van Dam P, Merajver S, Kleer CG, Harris AL, Van Marck EA, Dirix LY, Vermeulen PB

Publication: Breast Cancer Res Treat, 2004, Vol. 85, Page 13-22

PubMed ID: 15039594 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of staining TMAs rather than entire sections for the analysis of IBC and non-IBC specimens. The array contained 5 cores for each of 34 IBC cases and 3 cores for each of 41 non-IBC cases. ER and PR staining was scored on a scale of 0-3, HER-2 on a scale of 0-2, and p53 as positive (10% or more of tumor cells staining) or negative.

   Summary of Findings:

   In 17 of 34 IBC cases (50%), at least one of the 5 cores was non-informative due to specimen loss or lack of tumor cells, and 8 of the 34 (23.5%) cases had no informative cores. Only 2 of 41 non-IBC cases had non-informative cores, one of which had no informative cores. The number of informative cores was modestly but significantly lower among TMA sections that underwent heat-mediated antigen retrieval (30 minutes in a 98°C water bath) compared to those that did not (P<0.015). The concordance rates of ER, PR, HER-2 and p53 immunostaining in the TMA to those in the pathology reports based on whole section analysis were 0.74, 0.90, 0.85 and 0.50, respectively. A high degree of correlation in HER-2 staining was found between specimens stained using the HercepTest and the CB11, TAB250, or CB11 and TAB250 antibodies (k=0.86, 0.78, and 0.83, respectively). The TMA confirmed the differential expression of ER, PR, HER-2, p53, RhoC GTPase, and E-cadherin between IBC and non-IBC specimens, but it did not identify any differences in expression of EGFR or CA IX.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Protein	Tissue microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Type of slide	Whole tissue section Tissue microarray
   Immunohistochemistry Specific	Targeted peptide/protein	ER PR HER-2 p53 E-cadherin EGFR RhoC GTPase CA IX
   Immunohistochemistry Specific	Type of antibody	HercepTest TAB250 CB11 Both TAB250 and CB11
   Preaquisition	Diagnosis/ patient condition	Inflammatory breast cancer Non-inflammatory breast cancer
   Analyte Extraction and Purification	Antigen retrieval	30 min at 98 degrees C None Antigen retrieval solution

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