Comparison of frozen and RNALater solid tissue storage methods for use in RNA expression microarrays
Author(s): Mutter GL, Zahrieh D, Liu C, Neuberg D, Finkelstein D, Baker HE, Warrington JA
Publication: BMC Genomics, 2004, Vol. 5, Page 1471
PubMed ID: 15537428 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
To assess the contributions to genome wide variation of fixative type and duration (fresh, frozen, 24 h in RNAlater, 72 h in RNAlater) and inter-patient variability among the three cases examined, as measured by microarray.
Summary of Findings:
Of the 22,283 genes analyzed by microarray 7,853 varied among the experimental samples examined. The greatest source of variation was biospecimen source (inter-patient variability), with comparable levels of variation observed among replicates and fixation methods.
Biospecimens
Preservative Types
- RNAlater
- None (Fresh)
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform RNA DNA microarray Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Time in fixative 24 h
72 h
Biospecimen Preservation Type of fixation/preservation RNAlater
Snap frozen
No fixative