Comparison of frozen and RNALater solid tissue storage methods for use in RNA expression microarrays

Author(s): Mutter GL, Zahrieh D, Liu C, Neuberg D, Finkelstein D, Baker HE, Warrington JA

Publication: BMC Genomics, 2004, Vol. 5, Page 1471

PubMed ID: 15537428 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To assess the contributions to genome wide variation of fixative type and duration (fresh, frozen, 24 h in RNAlater, 72 h in RNAlater) and inter-patient variability among the three cases examined, as measured by microarray.

   Summary of Findings:

   Of the 22,283 genes analyzed by microarray 7,853 varied among the experimental samples examined. The greatest source of variation was biospecimen source (inter-patient variability), with comparable levels of variation observed among replicates and fixation methods.

   Biospecimens

   • Tissue - Uterus

   Preservative Types

   • RNAlater
   • None (Fresh)
   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   RNA	DNA microarray

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Time in fixative	24 h 72 h
   Biospecimen Preservation	Type of fixation/preservation	RNAlater Snap frozen No fixative

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