Transcriptional profiling of degraded RNA in cryopreserved and fixed tissue samples obtained at autopsy.

Author(s): Haller A C, Kanakapalli D, Walter R, Alhasan S, Eliason J, Everson R B

Publication: BMC Clin Pathol, 2006, Vol. 6, Page 9

PubMed ID: 17144909 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared the performance of snap-frozen and FFPE postmortem tissue on DASL multiplexed gene expression assays. Postmortem kidney, stomach, breast, lung, and brain were examined.

   Summary of Findings:

   Frozen specimens that yielded good quality RNA as measured on the Agilent bioanalyzer gave accurate and consistent results, as did the corresponding mirror-image, case-matched FFPE specimens. Frozen specimens with poor quality RNA also performed well in DASL assays; many but not all of the corresponding case-matched FFPE specimens likewise performed well in DASL assays. For FFPE specimens, highly expressed genes were more likely to give reproducible results than genes expressed at low levels.

   Biospecimens

   • Tissue - Kidney
   • Tissue - Brain
   • Tissue - Lung
   • Tissue - Stomach
   • Tissue - Breast

   Preservative Types

   • Frozen
   • Formalin

   Diagnoses:

   • Autopsy
   • Not specified

   Platform:

   Analyte	Technology Platform
   RNA	Automated electrophoresis/Bioanalyzer
   RNA	DASL

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Snap frozen
   Automated electrophoresis/Bioanalyzer Specific	Quality metrics	A score of "Passing" A score of "Failing"

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