Hematopoietic stem/progenitor cells, generation of induced pluripotent stem cells, and isolation of endothelial progenitors from 21- to 23.5-year cryopreserved cord blood.

Author(s): Broxmeyer HE, Lee MR, Hangoc G, Cooper S, Prasain N, Kim YJ, Mallett C, Ye Z, Witting S, Cornetta K, Cheng L, Yoder MC

Publication: Blood, 2011, Vol. 117, Page 4773-7

PubMed ID: 21393480 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of cryopreservation, freeze-thaw cycling, and storage of CB and CB-MNC on the recovery of HPCs and production of iPS cells. CB was assessed less than 36 h after collection and cryopreserved in 10% DMSO and 10% plasma as MNCs or unseparated CB.

   Summary of Findings:

   Compared to recovery rates from unpreserved specimens, less HPCs were recovered from cryopreserved MNCs (60-95%), regardless of storage duration, but recovery was highly variable. Further, the authors report >80% recovery of granulocyte, erythrocyte, monocyte, and megakaryocyte colony forming units (CFU-GEMM) and granulocyte and monocyte colony forming units (CFU-GM) from unseparated cord blood. Thawed specimens had HPC proliferation rates similar to fresh CB, and the HPCs were capable of being replated. Further, the authors report that 3 freeze-thaw cycles had no effect on HPC recovery. The rate of iPS cell generation from CD34+ cells was similar between thawed CB and fresh CB.

   Biospecimens

   • Bodily Fluid - Cord Blood
   • Cell - Cord Blood

   Preservative Types

   • None (Fresh)
   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	<36 h 10 years 15 years 21-23.5 years
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh)
   Storage	Freeze/thaw cycling	0 cycles 1 cycle 2 cycles 3 cycles

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