Red blood cell storage in SAGM and AS3: a comparison through the membrane two-dimensional electrophoresis proteome.
Author(s): D'Amici GM, Mirasole C, D'Alessandro A, Yoshida T, Dumont LJ, Zolla L
Publication: Blood Transfus, 2012, Vol. 10 Suppl 2, Page s46-54
PubMed ID: 22890268 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage solution and duration on the membrane protein profile of leukocyte-filtered RBC concentrates. Whole CPD blood was leukoreduced and suspended in SAGM, and whole CP2D blood from different donors was leukoreduced and suspended in AS-3. All RBC concentrates were stored at 1-6 degrees C and sampled aseptically on day 0, 21 and 42.
Summary of Findings:
After 21 days of refrigeration, 417 protein spots were found in the CPD-SAGM RBC concentrates which was 59% more than the 263 spots identified on day 0, but after 42 days of refrigeration, the number of spots identified in CPD SAGM RBC concentrates decreased to 345. On day 0, a comparable number of spots were detected in CPD-SAGM and CP2D-AS-3 RBCs (263 and 279, respectively). However, on day 21 and 42 of refrigerated storage, fewer protein spots were found in CP2D AS-3 RBCs than CPD-SAGM RBCs (312 versus 417 and 216 versus 345, respectively). Further, between days 21 and 42, the number of protein spots identified from refrigerated RBCs in CP2D-AS-3 decreased by 69%. During the first 21 days of storage, the high molecular weight proteins decreased while lower molecular weight proteins increased, with a larger shift observed in RBCs stored in CPD-SAGM than CP2D-AS-3.
Biospecimens
Preservative Types
- Other Preservative
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Protein 1D/2D gels Peptide 1D/2D gels Protein MALDI-TOF MS Peptide MALDI-TOF MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Refrigeration
None (fresh)
Storage Storage duration 0 days
21 days
42 days
Storage Short-term storage solution CPD-SAGM
CP2D-AS-3