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Red blood cell storage in SAGM and AS3: a comparison through the membrane two-dimensional electrophoresis proteome.

Author(s): D'Amici GM, Mirasole C, D'Alessandro A, Yoshida T, Dumont LJ, Zolla L

Publication: Blood Transfus, 2012, Vol. 10 Suppl 2, Page s46-54

PubMed ID: 22890268 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of storage solution and duration on the membrane protein profile of leukocyte-filtered red blood cell (RBC) concentrates.

Conclusion of Paper

On day 0, a comparable number of spots were detected in RBC concentrates stored in citrate-phosphate-dextrose (CPD) saline-adenine-glucose mannitol (SAGM) and those stored in CPD with a higher concentration of dextrose (CP2D)-additive solution 3 (AS-3). However, on day 21 and 42 of refrigerated storage, fewer protein spots were found in CP2D-AS-3 RBC concentrates than SAGM RBC concentrates. Between day 0 and 21 of storage, the number of protein spots identified from RBC concentrates increased, but the increase was much larger when the specimens were stored in CPD-SAGM than when stored in CP2D-AS-3, and the overall increase consisted of a decrease in high molecular weight spots and an increase in low molecular weight spots. Further, between days 21 and 42 of refrigerated storage, the number of protein spots decreased.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of storage solution and duration on the membrane protein profile of leukocyte-filtered RBC concentrates. Whole CPD blood was leukoreduced and suspended in SAGM, and whole CP2D blood from different donors was leukoreduced and suspended in AS-3. All RBC concentrates were stored at 1-6 degrees C and sampled aseptically on day 0, 21 and 42.

    Summary of Findings:

    After 21 days of refrigeration, 417 protein spots were found in the CPD-SAGM RBC concentrates which was 59% more than the 263 spots identified on day 0, but after 42 days of refrigeration, the number of spots identified in CPD SAGM RBC concentrates decreased to 345. On day 0, a comparable number of spots were detected in CPD-SAGM and CP2D-AS-3 RBCs (263 and 279, respectively). However, on day 21 and 42 of refrigerated storage, fewer protein spots were found in CP2D AS-3 RBCs than CPD-SAGM RBCs (312 versus 417 and 216 versus 345, respectively). Further, between days 21 and 42, the number of protein spots identified from refrigerated RBCs in CP2D-AS-3 decreased by 69%. During the first 21 days of storage, the high molecular weight proteins decreased while lower molecular weight proteins increased, with a larger shift observed in RBCs stored in CPD-SAGM than CP2D-AS-3.

    Biospecimens
    Preservative Types
    • Other Preservative
    • None (Fresh)
    Diagnoses:
    • Normal
    Platform:
    AnalyteTechnology Platform
    Protein 1D/2D gels
    Peptide 1D/2D gels
    Protein MALDI-TOF MS
    Peptide MALDI-TOF MS
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Biospecimen Preservation Type of fixation/preservation Refrigeration
    None (fresh)
    Storage Storage duration 0 days
    21 days
    42 days
    Storage Short-term storage solution CPD-SAGM
    CP2D-AS-3
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