Stability of serum carotene at various light and temperature conditions.

Author(s): Giasson J, Hernandez M, Chen Y

Publication: Arch Pathol Lab Med, 2011, Vol. 135, Page 1529-30

PubMed ID: 22129177 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage temperature and duration and exposure to light during storage on carotene levels in serum. Except for the serum measured on day 0, all serum was frozen after experimental storage until analysis.

   Summary of Findings:

   Carotene levels were 24.45% and 28.25% lower in specimens stored at room temperature for 10 and 14 days in the light, respectively than in specimens stored frozen at -70 degrees C for 14 days. Carotene levels were not significantly different after 14 days of storage at 4 or -30 degrees C or 14 days storage in the dark at room temperature compared to specimens stored at -70 degrees C for 14 days, nor were those measured in fresh specimens on day 0. A non-significant decrease in carotene levels of more than 10% was observed in specimens stored at room temperature in the dark for more than 7 days or in the light for more than 3 days.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)
   • Frozen

   Diagnoses:

   • Not specified
   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Freeze/thaw cycling	0 cycles 1 cycle
   Storage	Storage conditions	Exposed to light In the dark
   Storage	Storage temperature	-70 degrees C -30 degrees C 4 degrees C Room temperature
   Storage	Storage duration	0 days 1 day 3 days 7 days 10 days 14 days
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh)

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