Histomorphologic Assessment of Formalin Substitute Fixatives for Diagnostic Surgical Pathology

Author(s): Titford ME, Horenstein MG

Publication: Arch Pathol Lab Med, 2005, Vol. 129, Page 502

PubMed ID: 15794674 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To assess histomorphology after fixation with formalin and five formalin substitutes seven different surgical pathological tissues were evaluated: hepatocellular carcinoma, ovarian sex cord/stromal tumor, myxoid liposarcoma, uterine endometrioid adenocarcinoma, splenic follicular hyperplasia, infiltrating mammary carcinoma, and cecal signet ring carcinoma. Coded FFPE slides of each tumor were evaluated by four surgical pathologists in this blind study for the following histomorphologic parameters: cellular outlines, cytoplasmic detail, nuclear detail, erythrocyte integrity, lymphocyte integrity, overall morphology, and overall staining.

   Summary of Findings:

   While formalin fixation was paramount for overall morphology and staining, preservation of cellular outline, and erthrocyte integrity, nuclear features and lymphocyte detail were better preserved with Glyco-Fixx, and cytoplasmic detail was superior in Omnifix II fixed tissue.

   Biospecimens

   • Tissue - Liver
   • Tissue - Uterus
   • Tissue - Ovary
   • Tissue - Spleen
   • Tissue - Breast
   • Tissue - Colorectal
   • Tissue - Adipose

   Preservative Types

   • Formalin
   • Other Preservative

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Glyo-Fixx STF Omnifix II Histochoice Histofix Formalin (buffered)

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