Lysophosphatidylcholines: bioactive lipids generated during storage of blood components.

Author(s): Maslanka K, Smolenska-Sym G, Michur H, Wróbel A, Lachert E, Brojer E

Publication: Arch Immunol Ther Exp (Warsz), 2012, Vol. 60, Page 55-60

PubMed ID: 22167322 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of PCs at room temperature, storage of L and non-L-RBCs at 4 degrees C and using frozen apheresis plasma rather than fresh plasma to measure the levels of LysoPCs. Initial levels in each blood component were also compared. Non-L-RBCs were stored in citrate-phosphate-dextrose-adenine (CPDA), but L-RBCs were stored in Saline, Adenine, Glucose and Manitol (SAGM).

   Summary of Findings:

   The LysoPC content was comparable in fresh plasma from 10 donors, frozen apheresis plasma from a single donor and in the supernatant of PCs from 3 donors on day 0, but was lower in non-L and L-RBCs stored for 0-10 days at 4 degrees C (both p<0.05). L-RBCs had lower LysoPC levels than non-L-RBCs (p<0.05). LysoPC levels in the supernatant of PCs were higher after 1 day of storage at room temperature than after 0 days (p<0.05), but additional storage of PCs at room temperature did not lead to further increases in supernatant LysoPC content. During storage at 4 degrees C, LysoPC levels in non-L and L-RBCs decreased, often to below the limit of detection.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma
   • Cell - Red Blood Cells

   Preservative Types

   • None (Fresh)
   • Other Preservative
   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Lipid	HPLC

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	0 days 1 day 2 days 3 days 4 days 5 days 6 days
   Storage	Storage duration	0-10 days 11-20 days 21-30 days 31-40 days
   Biospecimen Aliquots and Components	Blood and blood products	Leukoreduced red blood cells Non-leukoreduced red blood cells Plasma Platelets
   Biospecimen Preservation	Type of fixation/preservation	Frozen None (fresh) Refrigeration

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