Immunohistochemistry on epoxy resin-embedded bone marrow biopsy experience with 936 cases.

Author(s): Tóth Z, Czoma V

Publication: Appl Immunohistochem Mol Morphol, 2011, Vol. 19, Page 15-20

PubMed ID: 20823769 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of epoxy resin embedding on IHC of bone marrow biopsies fixed in 10% formaldehyde. Specimens were compared to the authors previous experience with paraffin embedded specimens.

   Summary of Findings:

   Embedding bone marrow trephine biopsies in epoxy resin instead of paraffin allowed for visualization of detailed cytomorphology and immunomorphology in 3 um sections, eliminating the need for semithin technique. The process, from formaldehyde fixation to immunostaining, was completed in 3 days. IHC staining of epoxy resin embedded specimens with a panel of 40 to 50 primary antibodies was effective for the diagnosis of a range of lymphomas and leukemias, myelodysplastic syndromes, myeloproliferative neoplasms and non-malignant lesions and metastases.

   Biospecimens

   • Tissue - Bone Marrow

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal
   • Other diagnoses
   • Neoplastic - Benign
   • Neoplastic - Leukemia
   • Neoplastic - Lymphoma
   • Neoplastic - Other

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Hyperplasia Hypoplasia/aplasia Agranulocytosis Infection Granuloma Necrosis Autoimmune disease Chronic disease Toxic injury Virus-associated hemophagocytosis
   Biospecimen Preservation	Embedding medium	Epoxy resin Paraffin

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