Immunohistochemistry of Breast Tumor Markers on Archived Bouin-fixed Paraffin-embedded Tissues.

Author(s): Gagnon JF, Sanschagrin F, Jacob S, Provencher L, Morin C, Diorio C

Publication: Appl Immunohistochem Mol Morphol, 2011, Vol. 19, Page 168-72

PubMed ID: 20930615 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage and fixation method on IHC staining of breast cancer tissue specimens. Specimens included archived 10 year old BFPE and FFPE specimens, fresh BFPE and FFPE specimens, and 10 year old snap-frozen BFPE and FFPE specimens. IHC staining was completed on tissue microarrays containing all specimens.

   Summary of Findings:

   A concordance rate of 83.9% was observed between fresh-BFPE specimens and fresh-FFPE specimens when the positivity threshold of IHC staining was set at 10%. A concordance rate of 96.8% was achieved when positivity threshold of immunostaining was reduced to 3% to 4% for BFPE specimens. A positivity threshold of 10% led to a concordance rate of 88.5% for archived-FFPE and frozen-FFPE specimens, which was not changed when positivity thresholds were lowered. A concordance rate of 84.3% between archived-BFPE specimens and frozen-BFPE specimens was observed when the positivity threshold was set to 10% for both. When the positivity threshold was lowered to 4% to 6% for frozen-BFPE specimens, the concordance rate was 90.4%. A positivity threshold of 10% for frozen-FFPE specimens and 4% to 5% for frozen-BFPE specimens led to a concordance rate of 85.9%.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin
   • Other Preservative
   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Bouin's fixative Formalin (buffered) Frozen
   Storage	Storage duration	0 y 10 y
   Immunohistochemistry Specific	Technology platform	Dako Autostainer Plus Manual staining
   Immunohistochemistry Specific	Targeted peptide/protein	ER PR MSA CK 8/18 CK 19 p63 CK 5/6 CK 17 VIM EGFR CK 14 ECAD

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