Comparative analysis of detecting monocytic cells and their aberrancy.
Author(s): Dunphy CH
Publication: Appl Immunohistochem Mol Morphol, 2011, Vol. 19, Page 336-40
PubMed ID: 21293259 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare the detection of MCs in bone marrow by FC, IHC, and cytomorphology. Fresh peripheral blood specimens were also analyzed by FC.
Summary of Findings:
With a total of 40 specimens analyzed, more MCs were detected by FC than by cytomorphology in 45% of cases. A high concordance between the two detection strategies was seen 41% of the time. Hemodilution was shown for 5 FC specimens. FC was more sensitive for the detection of CD14, CD2, and CD56 than IHC, but IHC detection of CD14 was more specific for MCs. On the other hand, IHC detection of CD163, CD68, and CD33 showed low specificity for MCs. CD56 showed the highest correlation (93%) of immunostaining between clot and core biopsy specimens, while CD14 and CD33 showed more moderate correlations between the two specimen types of 85% and 70%, respectively. CD123, CD163, and CD68 showed greater immunostaining in clot sections than core biopsies, which the authors hypothesize was due to decalcification.
Biospecimens
Preservative Types
- Formalin
- None (Fresh)
- Other Preservative
Diagnoses:
- Neoplastic - Leukemia
Platform:
Analyte Technology Platform Cell count/volume Flow cytometry Protein Immunohistochemistry Morphology Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Immunohistochemistry Specific Targeted peptide/protein CD3
CD14
CD2
CD33
CD56
CD68
CD163
CD123
Biospecimen Preservation Type of fixation/preservation Air-dried
Formalin (buffered)
None (fresh)
Flow cytometry Specific Technology platform Immunohistochemistry
Cytomorphology (light microscopy)
Biospecimen Acquisition Method of tissue acquisition Core needle biopsy
Aspirate
Particle clot