Human postmortem brain tissue and 2-mm tissue microarrays.

Author(s): Kauppinen T, Martikainen P, Alafuzoff I

Publication: Appl Immunohistochem Mol Morphol, 2006, Vol. 14, Page 353

PubMed ID: 16932029 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to evaluate sample loss from tissue microarray slides when employing different slide types, antigen retrieval approaches, brain regions, and PMIs.

   Summary of Findings:

   PMI adversely influenced sample loss from microarray slides when pretreatment was not performed, although results were also dependent upon brain region and slide type (sample loss was more prevalent with ChemMate slides). Sample loss from slides was more frequent with longer PMI after autoclaving compared to microwave heating. Antigen retrieval solutions resulted in solution- and slide- dependent core sample loss: loss was more frequent with APES-coated slides) while treatment with 80% formic acid for up to 6 hours did not induce sample loss.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Formalin

   Diagnoses:

   • Alzheimer's Disease
   • Autopsy
   • Pneumonia/Respiratory Infection
   • Cardiovascular Disease

   Platform:

   Analyte	Technology Platform
   Protein	Tissue microarray
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Postmortem interval	16 h 96 h 278 h
   Biospecimen Aliquots and Components	Type of slide	Locally made; mechanical attraction SuperFrost Plus (Menzel-Glaser); electrostatic ChemMate (DakoCytomation); electrostatic
   Biospecimen Acquisition	Biospecimen location	Brain: gray matter Brain: white matter Brain: nuclei
   Analyte Extraction and Purification	Temperature of heat-induced retrieval	Microwave method Autoclave method

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2. Study Purpose

   The purpose of this study was to evaluate staining quality and reproducibility when employing different slide types, brain regions, and PMIs.

   Summary of Findings:

   The majority of core samples displayed appropriate and reproducible staining, although sample location on the slide impacted staining quality. Samples located in the corners of slides stained poorly compared to those in the center. A longer PMI positively influenced staining quality when two cases were compared (16 versus 96 h), although slide-dependent variability was also observed.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Formalin

   Diagnoses:

   • Alzheimer's Disease
   • Autopsy
   • Pneumonia/Respiratory Infection
   • Cardiovascular Disease

   Platform:

   Analyte	Technology Platform
   Protein	Tissue microarray
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Postmortem interval	16 h 96 h 278 h
   Biospecimen Aliquots and Components	Type of slide	Locally made; mechanical attraction SuperFrost Plus (Menzel-Glaser); electrostatic ChemMate (DakoCytomation); electrostatic
   Biospecimen Acquisition	Biospecimen location	Brain: gray matter Brain: white matter Brain: nuclei
   Tissue microarray Specific	Specimen position	Center of block/slide Periphery of block/slide

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