Immunohistochemistry of tissue prepared by a molecular-friendly fixation and processing system

Author(s): Nadji M, Nassiri M, Vincek V, Kanhoush R, Morales AR

Publication: Appl Immunohistochem Mol Morphol, 2005, Vol. 13, Page 277

PubMed ID: 16082256 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   Comparative analysis of Universal Molecular Fixative (UMFIX) and formalin using paraffin embedded tissues for the immunohistochemical detection of 70 commonly analyzed antigens.

   Summary of Findings:

   UMFIX-fixed tissue displayed comparable immunohistochemistry results to formalin fixed tissue for the majority of antibodies examined (42/70 antibodies), although superior (23/70) and inferior (5/70) staining was also observed. Inferior staining in UMFIX-fixed specimens was circumvented for 3 of the 5 antibodies by increasing the primary antibody incubation time. Further, antigen retrieval was not necessary in UMFIX-fixed specimens for 12 of the 70 antibodies tested, including the Hercep Test Kit.

   Biospecimens

   • Tissue - Prostate
   • Tissue - Skin
   • Tissue - Breast
   • Tissue - Testis
   • Tissue - Spleen
   • Tissue - Lung
   • Tissue - Liver
   • Tissue - Pancreas
   • Tissue - Brain
   • Tissue - Small Bowel
   • Tissue - Stomach
   • Tissue - Uterus
   • Tissue - Ovary
   • Tissue - Thyroid Gland
   • Tissue - Adenoid
   • Tissue - Thymus Gland

   Preservative Types

   • Formalin
   • Other Preservative

   Diagnoses:

   • Not specified - Benign
   • Not specified - Other

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Formalin (buffered) Universal Molecular Fixative

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