Effect of Prolonged Formalin Fixation on the Immunohistochemical Reactivity of Breast Markers
Author(s): Arber Daniel A
Publication: Appl Immunohistochem Mol Morph, 2002, Vol. 10, Page 183
PubMed ID: 12051639 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
To determine if prolonged formalin fixation affects the efficacy of immunohistochemical staining for the following breast cancer markers: estrogen receptor (ER), progesterone receptor (PR), c-erb-B2, Ki-67, p27, and vimentin.
Summary of Findings:
The immunoreactivity of ER, PR, and c-erb-B2 were significantly reduced after prolonged formalin fixation. Of the 33 cases examined, 8 displayed reduced immunostaining with prolonged fixation. Specifically, 3 of the 23 positive cases for ER overexpression displayed a significant reduction in staining after 57, 58, and 64 days of formalin fixation. Of the 21 positive cases of PR 1 displayed a significant reduction in staining after fixation for 120 days, and of the 9 positive cases for c-erb-B2 4 displayed a significant reduction in immunostaining after 20, 42, 99, and 120 days of fixation. Ki-67, p27, and vimentin expression were observed in all samples for all fixation times.
- Neoplastic - Carcinoma
Analyte Technology Platform Protein Immunohistochemistry
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Time in fixative 7-154 days