Expression of proliferative cell nuclear antigen (PCNA) in urinary bladder carcinoma. Evaluation of antigen retrieval methods.
Author(s): Malmström PU, Wester K, Vasko J, Busch C
Publication: APMIS, 1992, Vol. 100, Page 988-92
PubMed ID: 1361755 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine if paraffin block age affects PCNA immunoreactivity in formalin-fixed paraffin-embedded (FFPE) tissue specimens, and whether implementing an antigen retrieval technique can attenuate potential effects.
Summary of Findings:
PCNA immunostaining was well correlated to histological grade in FFPE sections from paraffin blocks less than 2 years old, while the extent and intensity of immunostaining progressively decreased as a function of block age in older specimens (3 years or older). Microwave heating FFPE sections in distilled water alone significantly improved PCNA immunostaining of older specimens, with the duration of heating proportional to the age of the paraffin block.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration < 2 y
3-6 y
> 10 y
Analyte Extraction and Purification Antigen retrieval Untreated
Microwave heating in distilled water
Pepsin (0.4%)
Neuroaminidase (0.02%)
Protease (0.1%)
Trypsin (0.1%)