Comparison of methods for collection of DNA samples by mail in the Black Women's Health Study.

Author(s): Cozier YC, Palmer JR, Rosenberg L

Publication: Ann Epidemiol, 2004, Vol. 14, Page 117-22

PubMed ID: 15018884 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare buccal cell collection by mouth swab or mouthwash with respect to DNA yield and PCR-based SNP analysis success rate. All specimens were collected by mail. Once received, mouth swabs were stored at -20 degrees C and mouthwash specimens were stored at 4 degrees C until DNA was extracted.

   Summary of Findings:

   Buccal cell DNA extracted from mouth swab specimens was slightly less contaminated with protein than that obtained from mouthwash specimens (p<0.001), as determined by OD 260/280 ratio. However, the average yield and concentration of DNA as determined by spectrophotometry from mouthwash specimens (30,179 ng/uL specimen and 509 ng/uL, respectively) were much greater than those from mouth swab specimens (4,344 ng/uL specimen and 66 ng/uL, respectively, p<0.001). PCR amplification of the NAT2-341 gene polymorphism was successful for 93% of mouth swab specimens and 94% of mouthwash specimens on the first try. A second attempt at PCR allowed for a 98% success rate from mouthwash specimens and 94% from mouth swab specimens.

   Biospecimens

   • Cell - Buccal
   • Bodily Fluid - Saliva

   Preservative Types

   • Frozen
   • Other Preservative

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	Spectrophotometry
   DNA	SNP assay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Mouth swab Swishing
   Biospecimen Preservation	Type of fixation/preservation	Frozen Refrigeration
   Biospecimen Acquisition	Method of cell acquisition	Mouth swab Swishing

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