Hemoglobin by first derivative spectrophotometry: extent of hemolysis in plasma and serum collected in vacuum container devices.

Author(s): Copeland BE, Dyer PJ, Pesce AJ

Publication: Ann Clin Lab Sci, 1989, Vol. 19, Page 383-8

PubMed ID: 2802517 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection tube and anticoagulant type, and collection volume on the amount of hemoglobin in serum and plasma specimens, as well as to determine the effects of storage of serum on the clot. Heparinated and citrated plasma and serum collected in plain tubes but not intended for storage on the clot were transferred to new plain tubes and stored at 4 degrees C for 1 to 7 days prior to analysis, but serum collected in SST was stored in the original container, and EDTA plasma was held at room temperature for 24-30 hours prior to analysis.

   Summary of Findings:

   On average, hemoglobin levels were significantly lower in plasma than in serum. Citrated plasma had significantly lower amounts of hemoglobin than heparinated or EDTA plasma. There were no significant differences between hemoglobin levels in serum stored in the original tube on the clot and serum transferred to new tubes for storage, or between serum collected in plain tubes and that collected in SST. Further, the size of the collection tube (and collection volume) did not affect hemoglobin levels among filled 5, 7, and 10 mL SST or 15 mL plain serum tubes.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)
   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Spectrophotometry
   Protein	Coulter counter

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Acquisition	Anticoagulant	Citrate EDTA Heparin None
   Biospecimen Acquisition	Type of collection container/solution	SST Plain serum tube
   Biospecimen Aliquots and Components	Aliquot size/volume	5 mL 7 mL 10 mL 15 mL
   Biospecimen Preservation	Type of fixation/preservation	None (fresh) Refrigeration
   Storage	Type of storage container	Original plain serum tube New plain serum tube

   View more