Human formalin-fixed paraffin-embedded tissues: an untapped specimen for biomonitoring of carcinogen DNA adducts by mass spectrometry.
Author(s): Yun BH, Rosenquist TA, Nikolić J, Dragičević D, Tomić K, Jelaković B, Dickman KG, Grollman AP, Turesky RJ
Publication: Anal Chem, 2013, Vol. 85, Page 4251-8
PubMed ID: 23550627 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of preservation method and detection method on quantification of deoxyadenosine (dA)-aristolactam (AL-I) adducts in upper urinary tract cancer specimens.
Conclusion of Paper
When analyzed by liquid chromatography mass-spectrometry (LC-MS), frozen and FFPE specimens had comparable levels of dA-AL-I adducts. For frozen specimens (FFPE was not investigated), the quantification of dA-AL-I adducts by LC-MS was comparable to that quantified by radioassay, but LC-MS was more sensitive.
Studies
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Study Purpose
The purpose of this study was to determine the effects of preservation method and detection method on quantification of dA-AL-I adducts in upper urinary tract cancer specimens. 5 specimens were immediately frozen at -80°C or fixed for 24 h in formalin. FFPE blocks were stored at room temperature. DNA was extracted from frozen specimens using a phenol-chloroform based method and from FFPE specimens using a modification of the ZR FFPE DNA MiniPrep kit.
Summary of Findings:
dA-AL-I adduct levels measured by LC-MS were comparable in frozen (0.13-7.0) and FFPE specimens (0.48-6.2). For frozen specimens (FFPE was not investigated), the levels of dA-AL-I adducts measured by LC-MS were comparable to those measured by radioassay, but LC-MS was more sensitive.
Biospecimens
Preservative Types
- Formalin
- Frozen
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform DNA Radioassay DNA LC-ESI-MS/MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Type of fixation/preservation Formalin (buffered)
Frozen
LC-ESI-MS/MS Specific Technology platform Radioassay