Immunostaining of estrogen receptor in paraffin sections of breast carcinomas using monoclonal antibody D75P3 gamma: effects of fixation.
Author(s): De Rosa CM, Ozzello L, Greene GL, Habif DV
Publication: Am J Surg Pathol, 1987, Vol. 11, Page 943-50
PubMed ID: 2825550 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of fixative type as well as duration and temperature of fixation on IHC staining of ER in breast tissue.
Summary of Findings:
For specimens fixed with Bouin's solution, optimum ER staining was observed after fixation for 1-2 h at room temperature. Longer fixation resulted in cytoplasmic background staining, loss of intensity, decreased sharpness of color, and/or less positively stained nuclei. Bouin's fixation at 4 degrees C gave poor results, regardless of time in fixative. However, formalin fixation for 1-2 h at 4 degrees C gave good results, while short room temperature formalin fixation did not. ER IHC staining and DCC assay results showed high concordance for Bouin's- and formalin-fixed specimens. A high correlation was also seen between use of the H222 antibody on frozen sections and use of the D75 antibody on paraffin sections.
Biospecimens
Preservative Types
- Formalin
- Frozen
- Other Preservative
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Protein Immunoassay Morphology Light microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Temperature of fixation/preservation Room temperature
4 degrees C
Biospecimen Preservation Time in fixative 30 min
1 h
2 h
4 h
6 h
24 h
Biospecimen Preservation Type of fixation/preservation Bouin's fixative
Formalin (buffered)
Frozen
Immunohistochemistry Specific Technology platform Immunoassay
Immunohistochemistry Specific Type of antibody H222
D75P3gamma