Effects of decalcification on immunoperoxidase staining.
Author(s): Mukai K, Yoshimura S, Anzai M
Publication: Am J Surg Pathol, 1986, Vol. 10, Page 413-9
PubMed ID: 2424325 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of decalcification solution and incubation time on IHC staining of 17 different tissues using a variety of antibodies.
Summary of Findings:
Neither histological structures nor immunostaining were significantly affected by decalcification, regardless of solution, as long as the incubation times were of standard lengths. Histological structures and immunostaining deteriorated after decalcification in formic acid beyond 1 week, EDTA after 4 weeks, nitric acid for 1 day, and Plank-Rychlo solution for 2 days. Declines in immunoreactivity were antigen specific with immunoglobulins being affected the most by prolonged decalcification.
Biospecimens
- Tissue - Tonsil
- Tissue - Appendix
- Tissue - Bone Marrow
- Tissue - Pituitary Gland
- Tissue - Thyroid Gland
- Tissue - Pancreas
- Tissue - Stomach
- Tissue - Small Bowel
- Tissue - Placenta
- Tissue - Prostate
- Tissue - Nerve
- Tissue - Muscle (Skeletal)
- Tissue - Brain
- Tissue - Esophagus
- Tissue - Bone
- Tissue - Lung
- Tissue - Blood Vessel
Preservative Types
- Formalin
Diagnoses:
- Not specified
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Morphology H-and-E microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Analyte Extraction and Purification Decalcification solution/ duration 5% formic acid
5% nitric acid
EDTA
Plank-Rychlo solution
None
Analyte Extraction and Purification Incubation duration/condition Decalcification
4 h
6 h
1 d
4 d
1 week
2 weeks
4 weeks