The effect of 96-hour formalin fixation on the immunohistochemical evaluation of estrogen receptor, progesterone receptor, and HER2 expression in invasive breast carcinoma.

Author(s): Yildiz-Aktas IZ, Dabbs DJ, Cooper KL, Chivukula M, McManus K, Bhargava R

Publication: Am J Clin Pathol, 2012, Vol. 137, Page 691-8

PubMed ID: 22523206 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare ER, PR, and HER2 IHC staining between core biopsies and whole sections of breast cancer tissue fixed in formalin for 96 h.

   Summary of Findings:

   Only minimal nonsignificant changes in semiquantitative scoring of ER, PR, and HER2 IHC staining were seen in both directions between core biopsies and 96 h-fixed tissue sections. The authors recommend that fixation times of up to 96 hours should be considered valid for all three antigens.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Morphology	H-and-E microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Time in fixative	96 h Core needle biopsy (unspecified)
   Biospecimen Acquisition	Method of tissue acquisition	Core needle biopsy Surgical resection

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