Fixation time does not affect the expression of estrogen receptor.
Author(s): Ibarra JA, Rogers LW, Kyshtoobayeva A, Bloom K
Publication: Am J Clin Pathol, 2010, Vol. 133, Page 747-55
PubMed ID: 20395521 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine if shortened fixation durations (1-9 h) of small tumor specimens (4 x 4 x 2 mm) impact the extent or intensity of estrogen receptor (ER) immunostaining. Of note, time in fixative does not reflect an additional 90 min in 20% formalin which occurred in a tissue processor.
Summary of Findings:
No differences in the percentage of immunopositive cells or staining intensity were observed among fixation durations (1-10 h) or the three anti-ER antibodies evaluated. However, all ten specimens examined were high ER expressing tumors.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Preservation Time in fixative 1 h
3 h
6 h
9 h
10 h