HER-2/neu Detection in Fine-Needle Aspirates of Breast Cancer: Fluorescence In Situ Hybridization and Immunocytochemical Analysis

Author(s): Beatty BG, Bryant R, Wang W, Ashikaga T, Gibson PC, Leiman G, Weaver DL

Publication: Am J Clin Pathol, 2004, Vol. 122, Page 246

PubMed ID: 15323142 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To assess HER-2 protein and gene amplification in formalin fixed paraffin embedded breast cancer tissue.

   Summary of Findings:

   HER-2 protein expression was elevated in 24% of the FFPE specimens analyzed, although only two-thirds of those cases also displayed elevated HER-2 gene expression.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	FISH
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)

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2. Study Purpose

   The purpose of this study was to assess HER-2 protein expression in cells collected by FNA and fixed using ethanol, formalin, or CytoLyt-ThinPrep.

   Summary of Findings:

   Results of HER-2 protein expression in FNA were fixative-dependent. Cells fixed in either formalin or CytoLyt-ThinPrep were moderately correlated to results obtained in FFPE tissue, while cells fixed in ethanol correlated poorly with data collected from FFPE tissue.

   Biospecimens

   • Cell - Breast

   Preservative Types

   • Formalin
   • Other Preservative
   • Ethanol

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Ethanol Formalin (buffered) CytoLyt-ThinPrep
   Biospecimen Acquisition	Method of tissue acquisition	Fine needle aspiration Surgical resection

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3. Study Purpose

   The purpose of this study was to assess HER-2 gene amplification via FISH in cells collected by FNA and fixed with ethanol or CytoLyt-ThinPrep.

   Summary of Findings:

   Results of FISH HER-2 gene analysis in FNA specimens fixed in ethanol or CytoLyt-ThinPrep were in strong agreement, and correlated well with HER-2 protein and gene expression data using FFPE tissues.

   Biospecimens

   • Cell - Breast

   Preservative Types

   • Ethanol
   • Other Preservative
   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   DNA	FISH

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Ethanol Formalin (buffered) CytoLyt-ThinPrep
   Biospecimen Acquisition	Method of tissue acquisition	Fine needle aspiration Surgical resection

   View more