Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effects of repeated freeze-thaw cycles on anticardiolipin antibody immunoreactivity.

Author(s): Brey RL, Cote SA, McGlasson DL, Triplett DA, Barna LK

Publication: Am J Clin Pathol, 1994, Vol. 102, Page 586-8

PubMed ID: 7942621 PubMed Review Paper? No

Suggested by: ISBER

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of freeze-thaw cycling on levels of anticardiolipin antibodies in spiked serum. Serum was stored at 4 degrees C overnight prior to spiking with IgG, IgA or IgM anticardiolipin antibodies and then freezing specimens. Serum was also stored at 4 degrees C after thawing at 37 degrees C until all specimens could be analyzed together.

   Summary of Findings:

   The mean IgG, IgA and IgM anticardiolipin levels in serum were lower after three freeze-thaw cycles compared to levels measured in refrigerated serum, but only the change in IgG anticardiolipin levels was significant (p<0.04). The largest decreases in anticardiolipin antibody levels occurred between 2 and 3 freeze-thaw cycles. Importantly, the changes in IgG and IgM anticardiolipin levels after 3 freeze-thaw cycles would have changed the diagnostic categories for 9 and 3 of 19 specimens, respectively.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Other Preservative
   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Freeze/thaw cycling	0 cycles 1 cycle 2 cycles 3 cycles
   Biospecimen Preservation	Type of fixation/preservation	None (fresh) Refrigeration

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