A novel method for optimum biopsy specimen preservation for histochemical and immunohistochemical analysis.

Author(s): Murray GI, Ewen SW

Publication: Am J Clin Pathol, 1991, Vol. 95, Page 131-6

PubMed ID: 1992605 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of freeze-substitution with low temperature plastic embedding, antibody incubation temperature and storage on histological and enzymatic analysis of biopsy specimens. All specimens were compared to authors expectations for formalin fixed wax embedded specimens.

   Summary of Findings:

   The authors report that freeze-substitution and plastic embedding led to better preservation of histochemical and IHC staining as well as enzymatic activity with low background and no diffusion observed. They report that antibody incubation at 37 degrees C, rather than room temperature, led to more consistent IHC results. Further, they report that freeze substituted plastic embedded specimens may be stored for 12 months at room temperature with no loss of immunoreactivity or enzyme activity.

   Biospecimens

   • Tissue - Colorectal
   • Tissue - Tonsil
   • Tissue - Muscle (Skeletal)

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified
   • Neoplastic - Carcinoma
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Protein	Enzyme assay
   Morphology	H-and-E microscopy
   Morphology	Light microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Type of fixation/preservation	Acetone Formalin (buffered)
   Immunohistochemistry Specific	Incubation temperature	37 degrees C Room temperature
   Storage	Time at room temperature	12 months
   Preaquisition	Diagnosis/ patient condition	Glycogen storage disease
   Biospecimen Preservation	Embedding medium	Glycol methacrylate Paraffin

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