Changes in serum chemical values as a result of prolonged contact with the clot.

Author(s): Laessig RH, Indriksons AA, Hassemer DJ, Paskey TA, Schwartz TH

Publication: Am J Clin Pathol, 1976, Vol. 66, Page 598-604

PubMed ID: 961639 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage of blood at room temperature prior to centrifugation on serum chemistry analytes. All specimens were kept out of direct light during storage.

   Summary of Findings:

   When blood was left at room temperature prior to separation of serum, glucose decreased by 30% after 24 hours and chloride decreased by 3% after 8 hours, but iron increased by 17% after 48 hours, potassium increased by 52% after 48 hours, and LDH increased steadily with levels 31% higher after 24 hours. Other clinical chemistry analytes including blood urea nitrogen, uric acid, creatinine, total bilirubin, inorganic phosphorus, calcium, cholesterol, magnesium, sodium, triglyceride, total protein, albumin, CPK, GPT, GOT, alkaline phosphatase, T3, and T4 were not affected by prolonged contact with the clot. Serum protein fractionation via electrophoresis showed no differences after 4 and 24 hours of storage at room temperature when compared to a standard 1 hour clot time.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)
   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer
   Protein	Electrophoresis
   Peptide	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	1 h 2 h 4 h 8 h 24 h 48 h
   Biospecimen Preservation	Type of fixation/preservation	Lyophilized None (fresh)
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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