Preparation of human whole lungs inflated and fixed for radiologic-pathologic correlation.

Author(s): Satoh K, Kobayashi T, Ohkawa M, Tanabe M

Publication: Acad Radiol, 1997, Vol. 4, Page 374

PubMed ID: 9156235 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to evaluate the morphology of postmortem lung tissue under varying inflation and formalin fixation conditions.

   Summary of Findings:

   The authors conclude that optimum morphology can be achieved in postmortem lung by inflation via cannulation of the main bronchus, formalin distension for at least 15 minutes, and expulsion of formalin with air, as opposed to manual expulsion, followed by application of the Heitzman method. Formalin fixation for longer than 15 minutes, up to 6 days, did not negatively impact inflation capability.

   Biospecimens

   • Tissue - Lung

   Preservative Types

   • Formalin

   Diagnoses:

   • Autopsy
   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	H-and-E microscopy
   Morphology	Ultrastructure

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Preservation	Time in fixative	15 min 30 min 2 h 2 d 4 d 6 d
   Biospecimen Acquisition	Cannulation	Inflated with cannulation Not inflated
   Biospecimen Preservation	Method of fixative removal	Manual explusion Expulsion via air HILUM rotation Expulsion via air STRING suspension

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