Peripheral Blood Mononuclear Cell (PBMC) and Associated Plasma Collection
BRD ID: 3,364
Source: NIAID Tier: SOP Number of times downloaded: 99
Description
This document describes procedures used by the Division of Microbiology and Infectious Diseases for the isolation, freezing, storage, and shipment of PBMCs for analysis of cell activation and cell-mediated immunity and humoral immunity. Separate procedures are described for (i) cell isolation from blood collected in Cell Preparation Tubes (CPT); (ii) cell isolation from blood collected in acid citrate dextrose (ACD), sodium heparin, or EDTA tubes with a pre-filled Frit Barrier; and (iii) cell separation from anticoagulated blood by Manual Density Gradient Media Overlay or Ficoll separation. It is a product of the National Institute of Allergy and Infectious Diseases (NIAID), Office of Clinical Research Resources (OCRR) and is available at https://www.niaid.nih.gov/research/dmid-specimen-resources.
Documents for Download
NIAID DMID -LB-SOP-00001-Peripheral Blood Mononuclear Cell (PBMC) and Associated Plasma Collection 9-19-2024.pdf [Size: 539 KB] Download SOP documents of all versions [2]Biospecimens
- Cell - White Cells
- Fluid - Blood
- Fluid - Plasma
Diagnoses
- General
Topics
- Biospecimen Processing
- Biospecimen Preservation
- Storage
- Biospecimen Shipment
Associated SOP Compendiums
- Biospecimen Processing
- Biospecimen Preservation
- Storage
- Biospecimen Shipment