NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

RKD Registry and Biobank: Sample Processing Lab Handbook   Add to my SOP compendium

BRD ID: 2,785

Source: RKD Biobank Tier: SOP Number of times downloaded: 1421

Description

This document describes procedures used by the Rare Kidney Disease Registry & Biobank and includes sample receipt by the laboratory; processing urine specimens for metabolomic analysis; processing blood collected into EDTA tubes for peripheral blood mononuclear cell (PBMC) isolation, proteomic analysis, and DNA extraction from plasma; and processing of serum specimens. Transportation of samples post-processing, sample storage, and emergency response in the event of a freezer malfunction are also addressed. It is a product of the Rare Kidney Disease Registry & Biobank in conjunction with the Vasculitis Ireland Network (VINE; https://www.tcd.ie/medicine/thkc/vasculitis/) and SOPs are available at https://www.tcd.ie/medicine/thkc/our-research/rita-ireland-vasculitis-riv-registry-and-biobank/.

Documents for Download

RKD Registry and Biobank-sample-process-lab-handbook-apr2020.pdf [Size: 618 KB]

Biospecimens
  • Fluid - Blood
  • Fluid - Serum
  • Fluid - Plasma
  • Cell - White Cells
  • Fluid - Urine
Diagnoses
  • Not specified
Topics
  • Biospecimen Processing
  • Storage
  • Biospecimen Shipment
  • Biospecimen Preservation

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