NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

Preparation of Colon Organoids   Add to my SOP compendium

BRD ID: 2,597

Source: UACC-TACMASR Tier: SOP Number of times downloaded: 1294

Description

This document describes instructions for collecting, processing, and cryopreserving normal and tumor colon tissue obtained by biopsy or resection and includes steps for thawing tissue to isolate cells and the subsequent culture and cryopreservation of colon organoids. This SOP is a product of the University of Arizona Cancer Center Tissue Acquisition and Cellular/Molecular Analysis Shared Resource (UACC-TACMASR) and is available at https://cancercenter.arizona.edu/researchers/shared-resources/tissue-acquisition-and-cellularmolecular-analysis.

Documents for Download

Preparation of Colon Organoids.pdf [Size: 4926 KB]

Biospecimens
  • Tissue - Colorectal
Diagnoses
  • Normal
  • Neoplastic - Carcinoma
Topics
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation

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