Preparation of Blood
BRD ID: 2,596
Source: UACC-TACMASR Tier: SOP Number of times downloaded: 1360
Description
This document describes instructions for isolating plasma from whole blood collected in EDTA tubes and serum and peripheral blood mononuclear cells (PBMCs) from whole blood collected in red top Vacutainer tubes and their subsequent storage. The protocols described are used by the UACC Biology Development and Research of organoids (BioDRoid) laboratory. Procedures for culturing dendritic cells, cytotoxic T-lymphocytes, and myeloid-derived suppressor cells from PBMCs and co-culture of organoid immune cells are also included. This SOP is a product of the University of Arizona Cancer Center Tissue Acquisition and Cellular/Molecular Analysis Shared Resource (UACC-TACMASR) and is available at https://cancercenter.arizona.edu/researchers/shared-resources/tissue-acquisition-and-cellularmolecular-analysis.
Documents for Download
Preparation of Blood.pdf [Size: 4932 KB]Biospecimens
- Fluid - Blood
- Fluid - Plasma
- Fluid - Serum
Diagnoses
- General
Topics
- Biospecimen Processing
- Storage
Associated SOP Compendiums
- Biospecimen Processing
- Storage