NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

IHMS SOP 06 V3: STANDARD OPERATING PROTOCOL FOR FECAL SAMPLES DNA EXTRACTION PROTOCOL Q   Add to my SOP compendium

BRD ID: 2,525

Source: IHMS Tier: SOP Number of times downloaded: 1882

Description

This document describes the protocol used to extract DNA from frozen fecal samples using the Qiagen QIASymphony DSP Virus/Pathogen Midi Kit and the QIASymphony SP instrument in order to characterize microbiota by metagenomic profiling. It is a product of the International Human Microbiome Standards (IHMS) project and is available for download at: http://mgps.eu/standard-operating-procedure/.

Documents for Download

IHMS_SOP_006_V3.pdf [Size: 1132 KB]
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Biospecimens
  • Fluid - Feces
Diagnoses
  • General
Topics
  • Biospecimen Processing
  • Analyte Extraction and Purification

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