NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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ISOLATION, CRYOPRESERVATION AND THAWING OF PERIPHERAL BLOOD MONONUCLEAR CELLS   Add to my SOP compendium

BRD ID: 2,464

Source: CAN-ASC Tier: SOP Number of times downloaded: 1559

Description

This document describes procedures for the collection of whole blood in EDTA or other blood collection tubes and the subsequent isolation, preservation, storage, and thawing of peripheral blood mononuclear cells (PBMC) and the processing, preservation, and storage of plasma. It is a product of the Canadian Autoimmunity Standardization Core (CAN-ASC) and is available at https://www.bcchr.ca/CAN-ASC/protocols.

Documents for Download

CAN-ASC_PBMC isolation, cryopreservation and thawing_20190321.pdf [Size: 300 KB]
Biospecimens
  • Fluid - Blood
  • Fluid - Plasma
  • Cell - White Cells
Diagnoses
  • General
Topics
  • Biospecimen Acquisition
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage

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