NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

SOP 2 - Solid Tumor Processing for Cell Culture and Xenografts   Add to my SOP compendium

BRD ID: 1,982

Source: Children's Oncology Group Tier: SOP Number of times downloaded: 1689

Description

This document describes the procedure used by the Childhood Cancer Repository and the Texas Cancer Cell Repository for processing tumor tissue for cell culturing, xenografts, and the establishment of cell lines. It includes steps for the culturing of steps, performing subcutaneous mouse injections, cryopreserving cells, maintaining cell cultures, and establishing cell lines. It is a product of the Children's Oncology Group (https://www.childrensoncologygroup.org/) and is available at: https://www.cccells.org/protocols.php.

Documents for Download

SOP2_Solid_Tumor_Processing.pdf [Size: 132 KB]

Biospecimens
  • Tissue - General
Diagnoses
  • Neoplastic - Carcinoma
Topics
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage

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