NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis

NCI BEBP: DNA and RNA Extraction from Formalin-Fixed, Paraffin-Embedded Tissue Biospecimens   Add to my SOP compendium

BRD ID: 1,901 Last Curated Date: 08-16-2018

Source: BBRB Tier: Biospecimen Evidence-Based Practices (BEBP) Number of times downloaded: 197


This evidence-based, expert-vetted best practice document is applicable for the extraction of nucleic acids from standard-size FFPE blocks of human tissue. DNA and mRNA extracted under these guidelines are suitable for downstream analysis by polymerase chain reaction (PCR), methylation analysis, Sanger and next-generation sequencing, and microarray hybridization. The BEBP document series is a product of the National Cancer Institute's Biorepositories and Biospecimen Research Branch (NCI BBRB) and can also be found on their website (

Documents for Download

Expert-vetted Nucleic Extraction from FFPE BEBP.pdf [Size: 438 KB]

  • Tissue - General
  • General
  • Analyte Extraction and Purification
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