NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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CHI Standard Operating Procedures for Isolation, Cryopreservation and Storage of Human Peripheral Blood Mononuclear Cells (PBMCs) and RNA   Add to my SOP compendium

BRD ID: 101

Source: CHI Tier: SOP Number of times downloaded: 1105

Description

This document describes the isolation, cryopreservation and storage of PBMC and the storage of PBMC lysates for subsequent RNA extraction. It is a product of the Center for Human Immunology, Autoimmunity and Inflammation (CHI; https://www.niaid.nih.gov/research/nih-center-human-immunology-inflammation-and-autoimmunity-chi) of the National Institutes of Health (NIH).

Documents for Download

SOP-Isolation.pdf [Size: 145 KB]
Biospecimens
  • Fluid - Blood
Diagnoses
  • General
Topics
  • Biospecimen Processing
  • Biospecimen Preservation
  • Storage
  • Analyte Extraction and Purification

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