Transitory interruption of recommended storage conditions does not cause significant changes in in vitro parameters of leucocyte-depleted red blood cells.
Author(s): Weiss DR, Fortenbacher S, Ringwald J, Strasser EF, Zimmermann R, Eckstein R
Publication: Vox Sang, 2011, Vol. 100, Page 272-8
PubMed ID: 20860559 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of the number of interruptions during refrigerated storage, storage duration and RBC processing method on the quality of RBCs in SAG-M. Both manufacturers produced RBCs from 490 mL citrate phosphate dextrose (CPD) whole blood. One manufacturer performed leukoreduction before RBC separation (whole blood filtration) while the other performed filtration on buffy-coat reduced RBCs (RBC filtration). Whole blood filtered RBCs had 0.02-0.04 mL/mL CPD, 0.2-0.33 mL/mL SAG-M and 0.05-0.13 mL/mL plasma, while RBC filtered RBCs had 0.006-0.010 mL/mL CPD, 0.27-0.45 mL/mL SAG-M and 0.024-0.04 mL/mL plasma. Further, RBCs were tested at the end of shelf life which was 42 days for whole blood filtered RBCs and 35 days for RBC filtered RBCs. RBC filtered units were tested again at 42 days. Interruptions to storage occurred when the blood was removed for cross-match (5-20 min at room temperature) or issuance or when specimens were returned from the intensive care unit or surgical wards (room temperature transport with several hours of refrigeration at destination).
Summary of Findings:
Up to 11 interruptions in refrigerated storage did not affect any of the blood quality parameters, and the authors report this held true regardless of whether the specimens were taken for cross-match (5-20 min at room temperature) or released and returned from another area of the hospital (unspecified time at room temperature during round-trip transit). However, whole blood filtered RBCs refrigerated for 42 days had higher MCV, hematocrit, concentration of hemoglobin, percentage thrombocytes, lactate, total hemoglobin, PO2, potassium, LDH, hydroxybutyrate dehydrogenase, and hemolysis and lower glucose, free hemoglobin, pH, and ATP than RBC filtered units refrigerated for 35 days. However, the difference in glucose content was attributed to the higher end concentrations of SAG-M in RBC filtered units than whole blood filtered units. When RBC filtered units were stored for 42 days instead of 35 days, the lactate, free hemoglobin, PO2, potassium, LDH, hemolysis and hydroxybutyrate dehydrogenase increased, and pH decreased. After 42 days of refrigeration, the potassium and pH were comparable in RBC filtered units and whole blood filtered units, but all other differences persisted. Further, while 7 of 43 whole blood filtered units had a >0.8% hemolysis after 42 days of refrigeration, none of the 24 and 26 RBC filtered units, refrigerated for 35 and 42 days, respectively, had >0.8% hemolysis.
Biospecimens
Preservative Types
- Other Preservative
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Cell count/volume Spectrophotometry Gas Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Electrolyte/Metal Ion selective electrode Protein Clinical chemistry/auto analyzer Protein Hematology/ auto analyzer Carbohydrate Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Small molecule Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 35 days
42 days
Storage Storage conditions 0 interruptions
1 interruptions
2 interruptions
3 interruptions
4 interruptions
5 interruptions
6 interruptions
7-8 interruptions
9-11 interruptions
Biospecimen Aliquots and Components Filtration Whole-blood filtration
RBC filtration
Biospecimen Aliquots and Components Blood processing method Whole blood filtration
RBC filtration