Storage induced apoptosis of peripheral blood mononuclear cells obtained from leucoreduction system chambers.

Author(s): Strasser EF, Weidinger T, Weiss DR, Strobel J, Zimmermann R, Eckstein R

Publication: Vox Sang, 2011, Vol. 101, Page 106-11

PubMed ID: 21492183 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of room temperature storage on cell counts and markers of apoptosis in PBMCs obtained using leucoreduction system chambers (LRSCs) from single and double Plt units. Platelets were collected by plateletpheresis with single units collected from donors with 160-230 x 10^9 platelets/L and double units from donors with >230 x 10^9 platelets/L. Buffy coats were stored in PVC bags for 72 h in a platelet incubator with repeat sampling from the same bag.

   Summary of Findings:

   Pre and post-donation WBC counts were higher in PBMC from double Plt units than single Plt units (p=0.047 and p=0.004, respectively), and they remained higher in PBMC from double Plt units throughout storage. The CD14+ monocyte and the percentages of annexin V and 7-AAD positive lymphocyte counts were higher in PBMC from double platelet units than single platelet units 1-48 h and 1-24 h after collection, respectively. Further, Plt counts, Plt volume, processed blood volume, and Plt yield were higher in PBMC from double Plt units than single Plt units. The WBC counts in PBMC were strongly correlated with processed blood volume (r2=0.75). During storage of PBMC at room temperature, CD14+ monocyte counts decreased significantly after 48 h (p<0.05), and the percentages of annexin V and 7-AAD positive lymphocyte counts increased by 17-25% over 72 h. The percentage of annexin V positive and 7-AAD negative monocytes decreased during the first 24 h and then increased during the remainder of the storage period, and the percentage of annexin V and 7-AAD positive monocytes decreased slightly during the first 6 h and then increased during the remainder of the storage period. The percentage of annexin V positive and 7-AAD negative lymphocytes and total percentage lymphocytes were not affected by storage or the use of a single versus double Plt units.

   Biospecimens

   • Bodily Fluid - Blood
   • Cell - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Cell count/volume	Flow cytometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	1 h 6 h 24 h 48 h 72 h
   Biospecimen Aliquots and Components	Aliquot size/volume	Single unit Double unit

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