Menopausal status affects the susceptibility of stored RBCs to mechanical stress.

Author(s): Raval JS, Waters JH, Seltsam A, Scharberg EA, Richter E, Kameneva MV, Yazer MH

Publication: Vox Sang, 2011, Vol. 100, Page 418-21

PubMed ID: 21488881 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of menopausal status, refrigerated storage duration and storage solution on MFI and hemolysis. RBCs preserved in AS-5 were collected locally and analyzed on day 7, 28, 42 and 49. RBCs in SAGM were shipped by courier from Germany to the United States on ice and analyzed on day 7, 21, 35 and 42. MFI was determined by measuring free hemoglobin before and after rocking with ball bearings. Results were compared with those from a previous study using specimens collected from men and pre-menopausal women.

   Summary of Findings:

   MFI increased significantly with refrigerated storage of AS-5 and SAGM-preserved RBCs (p<0.001) with a faster increase observed in SAGM than AS-5 specimens. Further, significant increases in MFI were noted between day 7 and 28 and day 28 and 42 in AS-5 RBCs (p<0.05, both) and between day 21 and 35 and day 35 and 42 in SAGM RBCs (p<0.01 and p<0.05, respectively). The storage-related increases in MFI observed in this study using RBCs from post-menopausal women in AS-5 or SAGM were comparable to those observed by the authors previously using RBCs in SAGM from men, but the increases were significantly higher than those observed by the authors previously using RBCs in SAGM or AS-5 from pre-menopausal women (p<0.05). Hemolysis increased slightly during refrigerated storage of RBCs in AS-5 and SAGM, but the increase was only significant in SAGM-preserved RBCs between day 21 and 25 (p<0.01). After 7 days of shipment on ice and/or refrigeration, hemolysis was comparable in locally collected AS-5 RBCs and shipped SAGM RBCs, which, the authors state, indicates no effect of shipment on hemolysis.

   Biospecimens

   • Cell - Red Blood Cells
   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	Spectrophotometry
   Cell count/volume	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	7 days 21 days 28 days 35 days 42 days 49 days
   Preaquisition	Patient age	>55 years Pre-menopausal
   Storage	Short-term storage solution	SAGM AS-5
   Preaquisition	Patient gender	Female Male
   Storage	Between site transportation method	Courier Unspecified transport method

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