The use of the mechanical fragility test in evaluating sublethal RBC injury during storage.

Author(s): Raval JS, Waters JH, Seltsam A, Scharberg EA, Richter E, Daly AR, Kameneva MV, Yazer MH

Publication: Vox Sang, 2010, Vol. 99, Page 325-31

PubMed ID: 20673245 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of patient gender, refrigerated storage duration and storage solution on MFI and hemolysis among RBCs. Leukoreduced RBCs preserved in AS-5 were collected locally and analyzed on day 7, 28, and 49, but leukoreduced SAGM RBCs were shipped from Germany to the United States on ice and analyzed on day 7, 21, 35 and 42. MFI was determined by measuring free hemoglobin before and after rocking specimens with ball bearings.

   Summary of Findings:

   MFI increased significantly with refrigerated storage of AS-5 and SAGM-preserved RBCs (p<0.001), although a faster increase in MFI was observed in SAGM specimens. Significantly lower MFIs were observed in AS-5 and SAGM RBCs from females than males. Hemolysis increased slightly during refrigerated storage of RBCs in AS-5 and SAGM, but the increases were only significant in AS-5-preserved RBCs between day 28 and 49 (p<0.05) and hemolysis remained at less than 0.8%. Blood type did not impact MFI, regardless of patient gender or storage solution.

   Biospecimens

   • Cell - Red Blood Cells
   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Spectrophotometry
   Protein	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	7 days 21 days 28 days 35 days 42 days 49 days
   Preaquisition	Patient gender	Female Male
   Storage	Short-term storage solution	SAGM AS-5
   Preaquisition	Patient genotype	Blood type O Blood type A Blood type B Blood type AB

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