Effects of handling and storage of blood on the stability of hepatitis C virus RNA: implications for NAT testing in transfusion practice.
Author(s): Grant PR, Kitchen A, Barbara JA, Hewitt P, Sims CM, Garson JA, Tedder RS
Publication: Vox Sang, 2000, Vol. 78, Page 137-42
PubMed ID: 10838513 PubMed Review Paper? No
Purpose of Paper
The purpose of this paperwas to determine the stability of hepatitis C virus (HCV) RNA in blood in the presence of different anticoagulants under a variety of storage temperatures and durations prior to processing.
Conclusion of Paper
HCV RNA levels declined significantly in blood specimens collected in tubes containing CPDA-1 and EDTA and stored at 37 degrees C for 120 hours. HCV RNA was stable at all other storage temperatures and durations.
Studies
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Study Purpose
The purpose of this study was to determine the stability of HCV RNA in blood in the presence of different anticoagulants under a variety of storage temperatures and durations prior to processing.
Summary of Findings:
HCV RNA levels declined significantly in blood specimens collected in tubes containing CPDA-1 and K3EDTA and stored at 37 degrees C for 120 hours. HCV RNA was stable at all other storage temperatures and durations.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Hepatitis
Platform:
Analyte Technology Platform RNA Real-time qRT-PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature 4 degrees C
25 degrees C
37 degrees C
Storage Storage duration 0 h
8 h
16 h
24 h
48 h
72 h
96 h
120 h
Biospecimen Acquisition Anticoagulant Plasma preparation tubes (PPT)
Citrate phosphate dextrose adenine 1
Potassium EDTA